The reaction was stopped with 10%aceticacidand washed with water.From theCambridge English Corpus
In the present study, however, the red/green fluorescence ratio significantly increased in zona-penetrated spermatozoa compared with zona-binding ones, following treatment withaceticacid.From theCambridge English Corpus
This method uses a mixture of anhydrousaceticacidand sulphuric acid, which is heated to prepare pollen for examination under a light microscope.From theCambridge English Corpus
Trematodes were fixed in glacialaceticacidand pressed between two slides, and preserved in 70% ethyl alcohol.From theCambridge English Corpus
When half of the thiocyanate solution was added, 25 ml of glacialaceticacidwas dropped and the solution was stirred for two more hours.From theCambridge English Corpus
The harvested eyes were placed in coldaceticacid/methanol solution overnight followed by paraffin embedding using standard procedures.From theCambridge English Corpus
A very small volume of fixative (methanol 3 :aceticacid1) was poured into the hypotonic solution.From theCambridge English Corpus
A freshly prepared mixture of 3 volumes methanol: 1 volumeaceticacidwas slowly run onto the pellet and poured off; this was repeated twice.From theCambridge English Corpus
If cytoplasm remained, it was removed by spreading one drop of another fixative, a mixture of methanol andaceticacid(3:1).From theCambridge English Corpus
The reaction was stopped in 10%aceticacid.From theCambridge English Corpus
Final preparation was carried out with a needle and dilutedaceticacid.From theCambridge English Corpus
Heptane-containing solutions with identical concentrations to those of theaceticacidand sodium hydroxide treatments were utilized with the same protocol as above.From theCambridge English Corpus
The repeated immersion technique was used (as in the bleach treatments described previously) with various total duration times of heptane andaceticacid.From theCambridge English Corpus
The stain was then differentiated withaceticacidand 70% ethanol to ensure staining of only the ganglion cell layer.From theCambridge English Corpus
Twenty-four hours after insemination, the oocytes were fixed in methanol/aceticacid(3 : 1, v/v) at room temperature and stained with 1% aceto-orcein.From theCambridge English Corpus
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